Fig. 4

High FAP expression predicts poor prognosis and promotes cell migration and invasion in NSCLC in vitro. a The FAP mRNA expression levels were significantly upregulated in 24 NSCLC tissues compared to the paired para-cancerous tissues. b FAP mRNA expression levels were significantly upregulated in NSCLC tissues. Data were obtained from the TCGA database. c Boxplot of FAP mRNA expression levels in NSCLC and normal tissues. Data were obtained from the HCMDB database (http://hcmdb.i-sanger.com/index). d FAP upregulation was associated with shorter overall survival (OS) times in NSCLC patients (P < 0.05). Data were obtained from the GEPIA database (http://gepia.cancer-pku.cn). e FAP mRNA and protein expression levels were significantly elevated in NSCLC cell lines. f KEGG analysis reflected that FAP overexpression is related to enrichment of pathways concerning cell metastasis. Data were obtained from the TCGA database. g The relative mRNA and protein expression levels of FTO in H460, H1299, and A549 cells. h–l Quantitative analysis of Transwell and wound healing assay data in H460, H1299, and A549 cells with FAP knockdown and FAP overexpression. m Western blot analysis verified that FAP regulated EMT-related proteins, including Snail, Slug, and N-cadherin, in NSCLC. Data information: Data are shown as the mean ± SDs. In all relevant panels, *P < 0.05; **P < 0.01; ***P < 0.001