Fig. 6

Indacaterol effect on SRSF6, ADRB2, integrin α5β1 and integrin αvβ3 expression in MDA-MB-231 cells. Kaplan–Meier plots presenting breast cancer patient survival in function of: A SRSF6 mRNA level, B SRSF6 mRNA level in low grades (I and II) and C. SRSF6 mRNA level in high grades (III and IV) from “Protein atlas data”; D mRNA levels of SRSF6, ADRB2, ITGA5, ITGB1, ITGAV and ITGB3 were measured by RT-qPCR after 24h and 48h of 15 µM indacaterol incubation, α-tubulin was used as house-keeping gene, results are expressed in fold change after being normalized to the corresponding untreated cells control; E Representative western-blot for SRSF6, ITGB3 and α-tubulin; F Quantification of protein abundance for SRSF6 and ITGB3 using α-tubulin charge as control (mean ± 1 SD of three independent experiments); Statistical significance was determined by two-way ANOVA (*p < 0.05; **p < 0.01; ***p < 0.001)