Fig. 5

Effect of indacaterol on collective and IGDQ-induced MDA-MB-231 cell migration. MDA-MB-231 cells were incubated or not with 15 µM of indacaterol in DMSO. A Cytonote holographic wound healing time-lapse imaging for 48h on control and indacaterol incubated cells; B Represented as the percentage of closed scratch along time, statistical significance was determined by two-way ANOVA (mean ± 1 SD of three independent experiments) (*p < 0.05; **p < 0.01; ***p < 0.001); C Represented by cell relative speed (relative distance per hour), statistical significance was determined by unpaired t-test with Welch’s correction (mean ± 1 SD of three independent experiments) (*p < 0.05; **p < 0.01; ***p < 0.001). D Ovizio holographic single IGDQ-induced cell migration on engineered surfaces, non incubated or incubated with 15 µM of indacaterol for 4 days; E SRSF6 (green) and phalloïdin (cytoskeleton – red) and nucleus (UV – Hoechst) immunofluorescent labeling of MDA-MB-231 cells grown on IGDQ-exposing surfaces for 5 days