Fig. 4

Validation of the inhibition of SRSF6 by Indacaterol in MDA-MB-231 cells. A Spliceosome complex SRSF6 dependent (adapted from Visconte and al [21].). U1 snRNP: splicing recruitment initiation protein; SRSF6: 5′splicing site serine-arginine rich (SR) protein regulating alternative splicing; SF1 U2 auxiliary factor subunit 2 U2AF2: bind the branch point (BP) sequence and the polypyrimidine tract (PT) on the intron; U2AF1: binds the AG dinucleotide, interact with U2AF2 and SRSF2 and recruitment of ZRSR2 and U2AF1L4 RNA-binding proteins; U2 snRNP: recruitment of SF3A1, SF3B1, and SAP130 to 3′splicing site. U4/U6 and U5 complex: recruitment of U1 and LUC7L2 at 5’ splicing site; PRFP8: catalyzation the release of the intron lariat and ligation of exons on BP and PT regions. *SAP130: Official gene name is SF3B3.; B ATXN2 pre-mRNA variants (NCBI—Gene ID: 6311); C MAP2 pre-mRNA representative lenght of the 52 variants (NCB1—Gene ID: 4133); D Ataxin-2 variants mRNA expression; E MAP-2 mRNAs global variants expression. mRNA levels of were measured by RT-qPCR after 24h and 48h of incubation with 15 µM indacaterol, α-tubulin was used as house-keeping gene, results are expressed in fold change after being normalized to the corresponding untreated control cells. Statistical significance was determined by two-way ANOVA (mean ± 1 SD of three independent experiments (# p < 0.05; **p < 0.01; ***p < 0.001). UD: undertermined (not or low expressed)