Fig. 6

Acetylsalicylic acid treatment reduces the secretion of multiple cytokines or growth factors related to the regeneration-associated gene network in vitro. The human growth factor/cytokine antibody array was analyzed using acetylsalicylic acid-treated or nontreated protein samples. The nitrocellulose membrane was spotted with specific antibodies for 40 different cytokines, growth factors, and receptors. Nine growth factors (AR, EGFR, GDNF, HB-EGF, IGFBP-6, M-CSF, PDGFRB, SCFR, and TGFß2) were clearly reduced in the protein samples from the acetylsalicylic acid-treated cells (A-B). The GEO metadata were also analyzed to further investigate the correlations between acetylsalicylic acid treatment and the nine downregulated proteins (C). All experiments were performed in triplicates. Significant differences are presented. *p < 0.05, **p < 0.005, and ***p < 0.001 (One-way ANOVA)