Fig. 7

Prx II re-expression inhibits exosome secretion by regulating ISGylation. A Prx II expression was assessed by immunoblotting in the three groups. Quantification of Prx II expression. B mRNA levels of ISG15 and Rab27b in NC and WT. (In Prx II ^−/− DMSCs, the group transfected with blank lentiviral vectors was denoted as the NC group, whereas the group transfected with Prx II lentiviral vectors was referred to as the WT group). C Protein levels of ISG15 and Rab27b in NC and WT. Quantification of ISG15 and Rab27b expression. D Quantification of UBA7, UBE2L6, and HERC6 mRNA levels in NC and WT cells via qRT-PCR. E Immunoblotting and quantification of UBA7, UBE2L6, and HERC6 protein expression. F Conjugated ISG15 levels in NC and WT. G Electron micrographs showing representative fields with MVBs (red arrows) in NC or WT. H Numbers of MVBs, ILVs, and ILV per MVB in over 20 fields per condition. I CD63 expression in WT and NC was determined by western blotting and quantification. *P < 0.05, **P < 0.01, ***P < 0.001 as determined by two-tailed t test