Fig. 6

Prx II depletion regulates Foxo1-induced miR-221 upregulation by inhibiting JNK activity. A Binding site of Foxo1 to the miR-221 promoter. B Expression of phospho-JNK in Prx II ^+/+ and Prx II ^−/− DMSCs. C Detection of JNK activation. D Foxo1 levels in the cell cytoplasm and nucleus were analyzed by western blotting after DMSCs were treated with 25 µM SP600125 for 48 h. Quantification of Foxo1 expression. E Expression of miR-221 of Prx II ^+/+ or Prx II ^−/− DMSCs treated with 25µM SP600125 for 48 h. *P < 0.05, **P < 0.01, ***P < 0.001 as determined by two-tailed t test