Fig. 3

Prx II depletion promotes exosome secretion by regulating ISGylation. A Heat map of proteins involved in exosome biogenesis or secretion identified via RNA-sequencing of Prx II ^+/+ and Prx II ^−/− DMSCs. Red and blue hues represent upregulated and downregulated mRNA, respectively. B mRNA expression of ISG15 and Rab27b in Prx II ^+/+ and Prx II ^−/− DMSCs was measured by qRT-PCR. C Protein expression of ISG15 and Rab27b was detected by western blotting of Prx II ^+/+ and Prx II ^−/− DMSCs. Quantification of ISG15 and Rab27b expression. D Proteins known to interact with ISGylation-related genes with high confidence are shown. The image was created using the STRING proteomics database. E qRT-PCR analysis of UBA7, UBE2L6, and HERC6 levels in Prx II ^−/− DMSCs and control cells, with β-actin as the control. F UBA7, UBE2L6 and HERC6 protein expression levels and quantitative analysis results G The expression of TSG101 in soluble and insoluble components level detection and quantitative. *P < 0.05, **P < 0.01, ***P < 0.001 as determined by two-tailed t test