Fig. 1

Prx II regulates exosome secretion in DMSCs. A Exosome size was analyzed by nanoparticle tracking analysis (NTA). Median statistics revealed that the diameter of Prx II ^+/+ DMSCs-Exos was 142.5 nm, while the diameter of Prx II ^−/− DMSCs- Exos was 152.5 nm. B NTA of the number of exosomes released by two cell types with equal cell volume. C The concentration of secreted protein was determined by bicinchoninic acid assay. D Western blot analysis of exosomes purified via serial ultracentrifugation of culture supernatants of equal numbers of Prx II ^+/+ and Prx II ^−/− DMSCs using the vesicular markers CD9 and HSP70. Quantification of protein contents in Prx II^+/+ and Prx II^−/−Exos. *P < 0.05, **P < 0.01, ***P < 0.001 as determined by two-tailed t test