Fig. 7
FTV inhibition alleviate renal interstitial fibrosis in diabetic mice. A The in vivo treatments plan. B1-B4 M0-ftv, M1-ftv, M2-ftv, HG-ftv, M1-ftvIL11- and HG-ftvIL11- were transferred into C57/BL6N mice kidney by renal parenchyma injection. Sham mice with PBS injection. Kidney samples were collected, renal tissue sections were stained with Masson’s trichrome, and α-SMA and COL1 in kidney samples was quantified using qPCR in each FTV group. n = 5. Scale bar: 50 μm. C Immunostaining images of CD68, CD11c, and CD206 in the renal interstitium of C57BL/6 N and STZ mice. n = 5. Scale bar: 50 μm. D1-D2 STZ mice were treated with CK636 or Dynasore. Tetraspanin 4 and integrin α5 in kidney samples detected by RT-qPCR, E1-E2 and renal tissue sections stained with Masson’s trichrome, E3-E4 immunostained with α-SMA in each group. n = 5. Scale bar: 50 μm.*P < 0.05 vs. the Sham group, the C57BL/6 N group. #P < 0.05 vs. the M1-ftv group, the HG-ftv group, the STZ group