Fig. 6

IL11 in HG/M1-ftv drives fibroblast transdifferentiation. A M1-ftv Gene ontology and KEGG pathways analysis. B The heatmap of FTV Gene profiling.C IL11 mRNA quantified using qPCR in each FTV group. n = 5. D1-D2 IL11 expression detected by Western blotting in each FTV group. n = 5. F Possible network of IL11. F1-F2 Immunofluorescence staining of fibroblasts treated with rmIL11 (5 ng/ml) to assess the expression of α-SMA and COL1. F3 MMP2 and TIMP1 mRNA quantified using qPCR in each FTV group. n = 3. Scale bar: 20 μm. G1-G4 Immunofluorescence staining of fibroblasts treated with M1-ftv, M1-ftvIL11-, HG-ftv, HG-ftvIL1-, or -combined with anti-IL11 antibody (2 μg/ml) to assess the expression of α-SMA and COL1. G5-G7 α-SMA and COL1 expression detected by Western blotting from each group. n = 3. Scale bar: 15 μm. α-SMA and COL1 (green), and cell nuclei stained with DAPI (blue). *P < 0.05 vs. the M0-ftv group, the unstimulated (--) group, the M1-ftv the, the HG-ftv group