Fig. 7

Recluster of myeloid cells and developmental trajectory of monocytes inferred by Monocle2. A TSNE plot of 9,179 myeloid cells reclustered, colored according to cell subtypes. B Percentage of each myeloid subtypes in different groups. Error bars represent mean ± SEM. Colored dots represent different samples. Differences with p < 0.05 were indicated; two-sided unpaired Wilcoxon rank sum test was used for comparison. C Developmental trajectory of monocytes and Mono-like MAC inferred by Monocle2, and the expression pattern based on the trajectory of genes of interest. D The most differentially enriched TFs between monocytes and Mono-like MAC investigated by SCIENIC algorithm (Left), the TFs exclusively enriched in each clustered were highlighted with black boxes. Expression of representative TFs (Right). Abbreviation: SCIENIC: Single-cell regulatory network inference and clustering; SEM: Standard error of mean; TFs: Translational factors; TSNE: T-distributed Stochastic Neighbor Embedding. * p < 0.05