Fig. 10

Ligand-receptor pairs-based cell–cell cross talk analysis. A The total number of in and out going signal from different cell types (Left). Representative circos plots showing top 15 most differentially expressed cytokine (Right Top) and growth factor (Right Bottom) L-R pairs compared between AIS/IAC and nLung. The red solid lines represent L-R pairs with higher expression in the AIS/IAC group compared to the nLung group, while the blue lines represent the opposite. The thickness of the lines represents the log2FC of ligands differential expression, and the size of the arrows represents the log2FC of receptor differential expression. The colors of the outer circle correspond to different cell types. B Expression pattern of CX3CL1, CX3CR1, CXCR3, and CCR7 in cell subtypes. C Screening of cytokine receptors on different cell types (Top). Expression of ligands for CCR4, CCR8, CD28 and SIRPG on different cell types (Bottom). D Expression of CCL2, CD80, CCL17 and CD86 in different clusters of DCs. E DEGs between Tregs with high and low expression level of SIRPG based on the mean expression level of SIRPG (Top left). Rests are the expression correlation of SIRPG with MHCII genes including HLA-DRA, CD74 and HLA-DPA1 in the LUAD cohort of TCGA. Abbreviation: AIS: Adenocarcinoma in situ; DEG: Differential expressed gene; FC: Fold of change; IAC: Invasive adenocarcinoma; LUAD: Lung Adenocarcinoma; TCGA: The Cancer Genome Atlas Program. * p < 0.05