Fig. 5
YTHDC1 recognizes differential m6A on PFKM and LDHA RNAs. A Schematic representation of m6A modification sites on mRNA of PFKM and LDHA. B The colocalization of YTHDC1 with PFK1M and LDHA was confirmed by RIP-qPCR. C, D The expression level of YTHDC1, PFK1M and LDHA were verified by qRT-PCR analysis and Western blotting in YTHDC1 knockdown cell lines. E The PFKM and LDHA mRNA half-lives were estimated according to linear regression analysis after the indicated actinomycin D treatment. Data are expressed as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001