Table 1 Primer sequences of target genes for reverse-transcription quantitative polymerase chain reaction
From: BRD4/nuclear PD-L1/RelB circuit is involved in the stemness of breast cancer cells
Gene name (human) | Primer sequences | |
|---|---|---|
Forward | Reverse | |
GAPDH | CACATGGCCTCCAAGGAGTAA | TGAGGGTCTCTCTCTTCCTCTTGT |
c-Myc | AATGAAAGGCCCCCAAGGTAGTTATCC | AGCAAAACCCGGAGGAGT |
CD44 | AGAAGGTGTGGGCAGAAGAA | AAATGCACCATTTCCTGAGA |
OCT4 | AGCAAAACCCGGAGGAGT | CCACATCGGCTGTGTATATC |
SOX2 | TTGCTGCCTCTTTAAGACTAGGA | CTGGGGCTCAAACTTCTCTC |
CD274 | AAAGTCAATGCCCCATACGG | TTCTCTTCCCACTCACGGGT |
RelB | GTCTTTCCCCACGAGGCTAT | CCGTACCTGGTCATCACAGAG |
IL-6 | ATGAACTCCTTCCTCCACAAGCGC | GAAGAGCCCTCAGGCTGGACTG |
IL-8 | CATACTCCAAACCTTTCCACCCC | TCAGCCCTCTTCAAAAACTTCTCCA |