Fig. 9

Effect of 3',4',7,8-tetrahydroxyflavone (THF) on tumor growth and immune response in mouse models. A In vivo experimental schedule. B Effect of THF of the mouse tumor. Mice were inoculated with MC38 cells and treated with THF. The body weight and tumor volumes of the mice were monitored for 25 days. After being sacrificed, tumor weight was determined. n = 5 in each group. C Protein expression regulation of THF in the tumor. The protein expressions of c-Myc, PD-L1, and RelB in tumors were detected by Western blot. D, E CSC marker, ALDH, expression of tumor or tumor-draining lymph nodes (TDLNs). Tumors or TDLNs were isolated into single cells, as described in the “Materials and methods” section. ALDH expression was measured using the ALDEFLUOR assay kit and a flow cytometer. F, G Helper T-cells or cytotoxic T-cells contained in the tumors and TDLNs. Tumors or TDLNs were isolated into single cells and analyzed using CD3⁺/CD4⁺ or CD3⁺/CD8⁺ staining, as described in the “Materials and methods” section. Compared with the control as determined by student’s t-test or one-way ANOVA with Dunnett’s multiple comparisons tests, *p < 0.05