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Fig. 10 | Cell Communication and Signaling

Fig. 10

From: Cardiac glycoside ouabain efficiently targets leukemic stem cell apoptotic machinery independent of cell differentiation status

Fig. 10

Ouabain induces a rapid loss of Mcl-1 and c-Myc in LSCs. A Kinetic analysis of Mcl-1 and c-Myc in enriched LSCs and LPCs from AML KG-1 cells following ouabain treatment (0 − 40 nM) for 24 − 72 h by Western blotting. β-actin was used as a loading control. Quantitative analysis of Mcl-1 and c-Myc levels is shown under the immunoblots. Dash line indicates juxtapose lanes taken from the same blots. B The half-life (t1/2) of Mcl-1 and c-Myc in enriched LSCs and LPCs upon ouabain treatment (40 nM) was determined in the presence of the protein synthesis inhibitor CHX (10 μg/mL)

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Cell Communication and Signaling

ISSN: 1478-811X