Fig. 4

 L-Fucose improved the proliferation and differentiation abilities of injured ENPCs in vitro. A Cell morphology of primary ENPCs at days 2, 4, and 7. B Immunofluorescence staining showed cells within neurospheres co-expressed Ngfr (green) and Nestin (red), but immuno-negative for glial cell marker GFAP (green) or neuronal marker PGP9.5 (red). By inducing differentiation, neurons (PGP9.5, red), glial cells (GFAP, green), and smooth muscle cells (α-SMA, gray) were derived from neurospheres; the nuclei (blue). C Cell viability of ENPCs treated with different concentrations of L-Fucose for 72 h was determined by CCK8 assay. D Ki67 staining of ENPCs in each group. E The positive rate of Ki67 was analyzed in the statistical chart. F Representative immunofluorescence images of ENPCs-derived neurons (HuC/D, green) or glial cells (GFAP, green) in each group; the nuclei (blue). G The positive rate of HuC/D and GFAP were analyzed in the statistical chart. H Western blot analysis of HuC/D and GFAP proteins in each group. I Densitometric analysis of HuC/D and GFAP proteins in each group. Con: Primary ENPCs cultures; Glu: ENPCs treated with 30 mM glucose; Fuc: ENPCs treated with 30 mM glucose and 30 mg/ml L-Fucose. ENPCs, enteric neural precursor cells. Results were expressed as mean ± standard deviation. *p < 0.05, ** p < 0.01, ***p < 0.001, ****p < 0.0001