Table 1 Oligonucleotide primers in real-time quantitative PCR
Species | Genes | Forward primer | Reverse primer |
|---|---|---|---|
Rat | StAR | GGGAGATGCCTGAGCAAAGC | GCTGGCGAACTCTATCTGGGT |
Rat | GR | CACCCATGACCCTGTCAGTC | AAAGCCTCCCTCTGCTAACC |
Rat | CTCF | ATGATCCCAACTTTGTCCCC | CCTCCATTTTCCCCTTCTACAC |
Rat | H19 | CCTCAAGATGAAAGAAATGGTGCTA | TCAGAACGAGACGGACTTAAAGAA |
Rat | Dnmt3a | GGTCATGTGGTTCGGAGATG | AGGACTTCGTAGATGGCTTTG |
Rat | Dnmt3b | TGGTAGGAGATGGAGATGGTG | AGATACTGTTGCTGTTTCGGG |
Rat | GAPDH | GCAAGTTCAATGGCACAG | GCCAGTAGACTCCACGACA |
Human | StAR | GGCATCCTTAGCAACCAAGA | TCTCCTTGACATTGGGGTTC |
Human | GR | CCCGTTGGTTCCGAAAATTG | AGCTTACATCTGGTCTCATGC |
Human | CTCF | CTGTTCCTGTGACTGTACCTG | AAAGGTAGGGTGTGGCATATC |
Human | H19 | GCACCTTGGACATCTGGAGT | TTCTTTCCAGCCCTAGCTCA |
Human | Dnmt3a | ATGGGCGTTAGTGACAAGAG | TCACAGTGGATGCCAACG |
Human | Dnmt3b | CCCATTCGAGTCCTGTCATTG | TTGATATTCCCCTCGTGCTTC |
Human | GAPDH | ACATCGCTCAGACACCATG | TGTAGTTGAGGTCAATGAAGGG |