Fig. 4

H19/let-7b axis mediated the effect of DEX on StAR expression in NCI-H295R cells. A Let-7b expression with H19 plasmid treatment; B StAR mRNA expression with H19 plasmid treatment; C StAR protein expression with H19 plasmid treatment; D H19 expression with let-7b inhibitor treatment; E StAR mRNA expression with let-7b inhibitor treatment; F StAR protein expression with let-7b inhibitor treatment; G H19 mRNA expression with CTCF plasmid treatment; H H19 mRNA expression with Dnmt3a siRNA treatment; I CTCF mRNA expression with GR siRNA treatment; J CTCF binding of H19 ICR with GR siRNA treatment; K Dnmt3a mRNA expression with GR siRNA treatment; L Dnmt3a binding of H19 ICR with GR siRNA treatment; M H19 mRNA expression with GR siRNA treatment; N Let-7b mRNA expression with GR siRNA treatment; O StAR mRNA expression with GR siRNA treatment; P Q GR, CTCF and StAR protein expression with GR siRNA treatment; R GRE in let-7b promoter region; S GR binding of let-7b. Mean ± S.E.M., n = 6 for RNA detection and n = 3 for western blot. ^*p < 0.05, ^**p < 0.01 vs. respective controls. DEX, dexamethasone; StAR, steroidogenic acute regulatory protein; CTCF, CCCTC binding factor; Dnmt3a, DNA methyltransferase 3a; GR, glucocorticoid receptor; GRE, glucocorticoid receptor element