Fig. 6

FBLN5 as synthetic lethality partner of MYC. a-d Neoplastic transformation of CV-1 cells upon stable overexpression of MYC. a Phase contrast micrographs of normal CV-1 cells and MYC-overexpressing CV-1 cells (CV-1 MYC). b Loss of contact inhibition in CV-1 MYC cells. Phase contrast micrographs of 3 weeks-old cell cultures. (a-b) Scalebar = 80 μm. c Cell cycle distribution of CV-1 normal cells and CV-1 MYC cells after PI staining and analysed by flow cytometry. d Growth curves of CV-1 normal cells and CV-1 MYC cells in serum-free medium. Cell proliferation was determined by MTT colorimetric assay with absorbance at 590 nm normalized to CV-1 normal cells at day 0. e Cell death in both normal CV-1 and CV-1 MYC cells upon FBLN5 transfection. Cells were harvested 48 h post-transfection, stained with PI and subjected to flow cytometry. Luciferase (luc) was used as a negative control and CAS8 was used as a positive control. f Cell death in MYC knockdown (MYC shRNA stable) MCF-7 cells upon FBLN5 transfection. MCF-7 cells stably transfected with scrambled shRNA were used as control (MCF-7 Control). Cells were harvested 72 h post-transfection, stained with Dioc₆ and PI and subjected to flow cytometry. Luciferase (luc) was used as a negative control and CAS8 was used as a positive control. Numerical values represent average ± SD of 3 independent transfections. Statistical significance was calculated using two-tailed Student’s t-test (*p ≤ 0.05, **p ≤ 0.005)