Fig. 4

Differential transcriptomic analysis of CV-1 and COS-7 upon FBLN5 experimental overexpression. a Schematic representation of the comparative transcriptomic analysis in CV-1 and COS-7 cells upon FBLN5 overexpression. Wild type and FBLN5 overexpressing CV-1 and COS-7 cells were subjected to microarray hybridization and subsequent differential gene expression analysis (DGE). Three sets of differentially expressed genes were obtained: CV-1 transfected with FBLN5 / wild type CV-1, COS-7 transfected with FBLN5 / wild type COS-7 and wild type COS-7 / wild type CV-1. These three sets were subjected to the Ingenuity Pathway Analysis (IPA) Core Analysis individually. The three core analysis reports thus obtained were subjected to the IPA Comparison Analysis. As an additional step, the two differentially expressed sets of genes (CV-1 transfected with FBLN5 / wild type CV-1 and COS-7 transfected with FBLN5/ wild type COS-7) were also compared separately using Venn diagram. b Heatmap of 20 most differentially expressed genes between FBLN5-transfected COS-7 (left) and CV-1 (right) cells vs wild type COS-7 and CV-1 cells respectively. Heatmap filters, COS-7 cells: -8.5 ≤ fold change ≥ 7, p ≤ 0.05, FDR ≤ 0.05, CV-1 cells: -10.8 ≤ fold change ≥ 13.5, p ≤ 0.05, FDR ≤ 0.05. c Types of differentially expressed transcripts in COS-7 cells transfected with FBLN5 / wild type COS-7 cells (top), CV-1 cells transfected with FBLN5 / wild type CV-1 cells (mid) and wild type COS-7 cells / wild type CV-1 cells (bottom). Filter: -2 ≤ fold change ≥ 2, p ≤ 0.05, FDR ≤ 0.05. d Comparative Venn diagram of differentially expressed genes in CV-1 cell (blue) and COS-7 (yellow) upon FBLN5 transfection, only 20.1% differentially expressed genes were common among the two differentially expressed gene sets