Fig. 1

Schematic representation of the screening for anticancer genes through robotic single cDNA investigation (RISCI) process. a Employing robotic automation, plasmids are propagated in E. coli, DNA isolated and transfected in mammalian cells grown in 96-well plates with one gene per well and assayed for the desired phenotype (grey mask) — frequently cell death. b Cell death inducing genes in HEK293T cells were isolated through RISCI. These genes were transfected into untransformed CV-1 cells and a subset, which did not cause cell death, was identified as anticancer genes. One of the anticancer genes identified, FBLN5, was also transfected into MYC-transformed CV-1 cells resulting in cell death