Fig. 7

E6446 prevents HFD-induced NAFLD. C57BL/6J mice were fed a HFD for 12 weeks and supplemented with E6446 (20 mg/kg) or vehicle three times per week for 6 weeks. The mice were finally sacrificed, and further analyses were performed as indicated below. A Schematic of the experimental strategy. B Body weight. C Liver weight. D WAT weight and Fat/body ratio. E GTT. The hepatic (F) and serum (G) levels of TC, TG, LDL, ALT, and AST (n = 6). H Representative H&E (upper) and Oil red O (lower) staining of the liver sections of the mice in the indicated group. The values presented are the means ± SEMs. Scale bars, 50 μm. *P < 0.05, **P < 0.01, ***P < 0.001 vs. the normal control diet (NCD) group. ^#P < 0.05, ^##P < 0.01, vs. the HFD group