Fig. 6

ATF3 silencing blocks E6446-induced inhibition of adipogenic differentiation and hepatic lipogenesis. OP9 cells were transfected with ATF3 siRNA or control siRNA and 24 h later, the cells were incubated with 1 μM rosiglitazone for 15 days to induce adipogenic differentiation. Effect of E6446 (10 μM) on A TG level, B mRNA expression of adipogenic differentiation-related genes, and C Oil Red O staining (scale bar: 200 μm, 250 μm). After transfection foe 24 h, AML12 cells were treated with a combination of palmitic and oleic acid (PAOA) for 48 h. Effect of E6446 (10 μM) on D TG level, E mRNA expression of lipogenesis-related genes, and F Oil Red O staining (scale bar: 200 μm, 250 μm). The values presented are the means ± SEMs of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 vs. the Control group. ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. the E6446 group