Fig. 4

E6446 is more potent and safer than A939572 in preventing adipogenic differentiation and hepatic lipogenesis. The effect of different concentrations of E6446 and A939572 on LDH release and cellular viability in OP cells (A-B) and AML12 cells (C-D). For adipogenic differentiation, OP9 cells were incubated with 1 μM rosiglitazone for 15 days to induce adipogenic differentiation. The effect of E6446 and A939572 on E cellular TG level, F mRNA expression of adipogenic differentiation-related genes, and G Oil Red O staining (scale bar: 200 μm, 250 μm). During lipogenesis, AML12 cells were incubated with E6446 (10 μM) or A939572 (10 μM). The effect of E6446 and A939572 on H cellular TG level. I mRNA expression of lipogenesis-related genes, and (J) Oil Red O staining (scale bar: 200 μm, 250 μm). K Biacore X100 detected the kinetics/Affinity of E6446 and A939572 with SCD1. The values presented are the means ± SEMs of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 vs. the Control group. ^#P < 0.05, ^##P < 0.01, vs. E6446 group