Fig. 2

E6446 prevents adipogenic differentiation and hepatic lipogenesis. For adipogenic differentiation, OP9 cells were incubated with 1 μM rosiglitazone for 15 days to induce adipogenic differentiation. Schematic of the adipogenic differentiation (A) and hepatic lipogenesis (B) strategy. Effect of five potential compounds (10 μM) on the C cellular triglyceride (TG) level, D mRNA expression of adipogenic differentiation-related genes, and E Oil Red O staining (scale bar: 200 μm, 250 μm). For lipogenesis, AML12 cells were treated with a combination of palmitic and oleic acid (PAOA) for 48 h. Effect of five potential compounds (10 μM) on the F TG level, G mRNA expression of hepatic lipogenesis-related genes, and H Oil Red O staining (scale bar: 200 μm, 250 μm). The values presented are the means ± SEMs of three independent experiments. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 vs. the Control group