Fig. 8

Representative images of DAPI stained nuclei of T24 cells and fibroblasts (A, B). Nuclear morphology profiles of T24 (C) and bladder fibroblasts (D), shown are the average values of Rel. Area (Area [µm]/200), roundness, aspect ratio, circularity and solidity (complete list of the average values and standard deviation can be found in supplementary table 1). In total n = 12 optical fields were evaluated, and the average values of all nuclei per image were calculated, for 3 independent cell preparations (biological replicates). Representative images of actin (blue-white) and nuclei (orange) in T24 cells (E) and fibroblasts (F). Quantification of actin in T24 cells (G) and fibroblasts (H). N ≥ 36 ROIs for T24 and n ≥ 26 ROIs for fibroblasts were evaluated for each respective subcellular compartment, for 3 independent cell preparations (biological replicates). Quantification and representative images of the distance between actin (phalloidin, blue-white) and nuclear (DAPI, orange) signals for T24 (I) and fibroblasts respectively (J). Graphical representation of selected cross-sections, showing the signal intensity (r.f.u.) over the distance [µm]; highlighted is the measurement of the gap between the nuclear and actin signals (dotted line marks reference threshold of 50 r.f.u.). In total n ≥ 72 ROIs for T24 and n ≥ 54 for fibroblasts were evaluated, for 3 independent cell preparations (biological replicates). */§ Indicates statistical significance at Student´s t-test (*: p < 0.05; **/§§: p < 0.01; §§§: p < 0.001)