Fig. 7
Response of T24 and fibroblasts to short term (1-2 h) incubation with 100nM TG. Quantification of the ER appearance for T24 cancer cells (A) and fibroblasts (C) respectively, results are given as mean ER footprint area per cell [µm2] (average per optical field). * Indicates statistical significance at Mann–Whitney test (n.s. p > 0.05; **: p < 0.01; ***: p < 0.001). Results were taken from 3 independent cell preparations and n ≥ 16 optical fields were evaluated. Representative images of T24 cells (B) and fibroblasts (D) before treatment (PRE) and after 30, 60, and 120 min incubation with 100nM TG. ER is depicted in green. Representative AFM height and Young´s modulus maps of controls and 100nM TG treated cells (1-2h), T24 (E) and fibroblasts (F). Quantification of the Young´s modulus per cell in the nuclear (NUC), perinuclear (PERI) and cytoplasmic (CYT) area, for both T24 cells (G) and fibroblasts (H). */§ Indicates statistical significance at Mann–Whitney test (*/§: p < 0.05; **/§§: p < 0.01; ***: p < 0.001) § indicates differences between controls and cells treated with TG 100nM, * indicates any remaining significances. Results are taken from 3 independent cell preparations from n ≥ 17 cells