Fig. 5

A Measurement of the Young´s modulus (YM) at the nuclear (a), perinuclear (b) and cytoplasmic region (c). B Quantification of the YM [kPa] per ROI of n ≥ 13 cells per condition. Taken from n ≥ 3 cell preparations (biological replicates). Nuclear (a, NUC), perinuclear (b, PERI) and cytoplasmic (c, CYT) areas were evaluated. Statistical significance is show at Mann-Whitney test, */§ indicates significant difference between treatments (*/§: p ≤ 0.05; **: p ≤ 0.01; ***: p ≤ 0.001). C Representative distribution of the Young´s modulus curves in 3.1 µm × 3.1 µm square ROIs, selected for each treatment and cell region (nuclear, perinuclear and cytoplasmic)