Fig. 3

A Fluorescence staining of actin (blue to white) and the nucleus (orange) in T24 cells after 24 h incubation in control conditions and with 20nM BFA and 1nM TG as well as after co-incubation with 100nM CytD. B Quantification of actin signal intensity in cytoplasmic and nuclear region of T24 cells as relative fluorescent units (r.f.u.) after 24 h incubation in control conditions, BFA, TG and their combination with CytD. Grey symbols: § indicates significant difference between control incubations with and without CytD. Yellow symbols: § indicates significant difference between TG incubations with and without CytD. Cyan symbols: § indicates significant difference between BFA incubations with and without CytD (§§§: p ≤ 0.001). Black symbols: * indicates significant difference between treatments (*: p ≤ 0.05; **: p ≤ 0.01). Data were acquired from 3 independent cell preparations n > 70 ROIs. C Representative images of the gap-closure migration assay at t = 0 and t = 24 h. Control cells as well as 24 h incubation with 20nM BFA and 1nM TG. D Quantification of the cell migration results are given as area healed, n.s. denotes no statistical significance (p > 0.05) at Mann-Whitney test. Data were acquired from 4 independent cell preparations, n ≥ 11 optical fields were evaluated