Fig. 2

A Representative images of the immunofluorescence staining of IRE1 (red), actin (blue to white) and the nucleus (orange) of T24 cells after 24 h incubation in control conditions and with 20nM BFA and 1nM TG as well as after co-incubation with 100nM CytD. B Quantification of IRE1 signal intensity in cytoplasmic and nuclear region of T24 cells as relative fluorescent units (r.f.u.) after 24 h incubation with BFA, TG and their combination with CytD. Yellow symbols: § indicates significant difference between TG incubations with and without CytD. Cyan symbols: § indicates significant difference between BFA incubations with and without CytD (§: p ≤ 0.05; §§: p ≤ 0.01; §§§: p ≤ 0.001). Black symbols: * indicates significant difference between treatments (*: p ≤ 0.05; **: p ≤ 0.01). Data was acquired from 3 independent cell preparations n > 70 ROIs. C Representative image of DAPI (Cyan) stained nuclei of T24 cells after 24 h incubation in control conditions and TG treatment. D Quantification of nuclear circularity, statistical significance to control is at Student´s t-test (**: p ≤ 0.01) yellow § indicates difference between TG and TG in combination with CytD. Data were acquired from 3 independent cell preparations n > 50 cells. E Quantification of nuclear area results, statistical significance to control at Student´s t-test (***: p ≤ 0.01) grey and cyan §§§ indicates difference between controls/BFA and controls/BFA in combination with CytD. Data were acquired from 3 independent cell preparations n > 50 cells