Fig. 7
From: Castration promotes the browning of the prostate tumor microenvironment
Effect of 3T3-L1 secretome on the proliferation of PCa cells in vitro. A Edu labeling of proliferating TRAMP-C1 cells after co-culture assay in Transwell.™ inserts for 48 h with 3T3-L1 undifferentiated cells (naïve), differentiated in presence of androgen signaling (DMSO) or without androgen signaling (CDX). Images were taken at 100X of magnification. B Quantification of EdU positive cells vs DAPI staining cells in the same groups as previously described. C TRAMP-C1 cell number after incubation 48 has the same condition in the previous graph. D Cell number quantification of TRAMP-C1, LNCaP and PC3 cells incubated with 3T3-L1 derived EVs isolated from undifferentiated fibroblast or differentiated in presence of DMSO or CDX. n = 3. Data were represented as mean ± SEM. * p < 0.05, ** p < 0.01