Fig. 5
From: Castration promotes the browning of the prostate tumor microenvironment
Castration induces PPAT browning, reduces macrophage infiltration. A Toluidine blue stain of slides from 24 weeks old WT and TRAMP mice after 12 weeks of castration. Arrows shows metachromatic cells (mast cell) in the prostate stroma (left panel). Images were taken at 400X of magnification. Mast cell quantification per total gland surface (right panel). B Macrophages detection in the prostate stroma from 24 weeks old WT and TRAMP mice after 12 weeks of castration (left panel). Arrows shows F4/80 positive cells. Quantification of F4/80 surface per DAPI surface (right panel). Arrows shows F4/80 positive cells. Quantification of F4/80 surface per DAPI surface (right panel). For all quantifications, 5 different images from each mouse were analyzed. n = 5. Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001