Fig. 2

Inhibition of nSMase differentially modulates small and large EVs. A Schematic of workflow to obtain 10,000 × g and 100,000 × g sediments from hRGC supernatant. B 100,000 × g sediment contain smaller particles versus 10,000 × g sediment in both (left) DMSO- and (right) GW4869-treated cultures. Asterisks indicate p ≤ 0.0451. C Quantification of particle analysis of 10,000 and 100,000 × g pellets from supernatant of DMSO- and GW4869-treated cells. N = 3 independent samples from each condition. Asterisks indicate p ≤ 0.0115. Statistics: B, C Two-way repeated measures ANOVA, Bonferroni post hoc tests. Data presented as mean ± sem