Fig. 6
RIPK1-mediated ASK1 activation promotes NEK7/NLRP3 activation in Caspase 6-controlled inflammatory response in macrophages. BMMs were isolated from Caspase 6.KO and WT mice and transfected with the p-CRISPR-RIPK1 activation, p-CRISPR-RIPK1 KO or control vector followed by LPS stimulation. A D Western blotting and relative intensity of RIPK1, p-ASK1, NEK7, NLRP3, and C-caspase-1, N = 6/group. B E Immunofluorescence staining for the NLRP3 expression in macrophages. DAPI was used to visualize nuclei. Scale bars: 20 μm, 10 μm, N = 4/group. C F ELISA analysis of IL-1β levels in culture medium, N = 6/group. All data represent the mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001