Fig. 5
NEMO mediated RIPK1-IκBα interaction induces RIPK1 degradation while inhibited NEK7/NLRP3 function in macrophages. Bone marrow-derived macrophages (BMMs) derived from Caspase 6KO mice were transfected with NEMO siRNA for 24 h followed by LPS treatment for 6 h. Immunofluorescence staining of IκBα (A) and RIPK1 (B) in LPS-stimulated macrophages. DAPI was used to visualize nuclei (blue). Scale bars: 20 μm, N = 4/group. C-D Immunoprecipitation analysis of RIPK1 and p-IκBα in LPS-stimulated macrophages from WT and Caspase 6.KO mice, N = 5/group. E F Western blotting analysis and relative intensity of p-IκBα, IκBα, RIPK1, NEK7 and NLRP3, N = 6/group. All data represent the mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001