Fig. 3
Disruption of Caspase 6 regulates RIPK1/IκBα signaling and inhibits NEK7/NLRP3 activation and ferroptosis in IR-stressed livers. Western-assisted analysis and relative density ratio of (A) NEMO, p-IκBα, IκBα, RIPK1 in Sham and IR groups; (B) RIPK1, NEMO, p-ASK1 in the WT and Caspase 6KO mice after liver IR; (C) NEMO and p-ASK1 in Kupffer cells and hepatocytes isolated from ischemic livers; (D) NEK7, NLRP3, and cleaved caspase-1 in the ischemic livers, N = 6/group. E ELISA assay of serum HMGB1 in the WT and Caspase 6KO mice after liver IR, N = 6/group. F The expression and relative intensity of NOX1, ACSL4 and GPX4 in IR-induced WT and Caspase 6KO livers, N = 6/group. G IHC staining of ACSL4 expression in IR-induced livers, scale bar: 50 μm, N = 4/group. All data represent the mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001