Fig. 2
Disruption of Caspase 6 alleviates IR-induced liver injury, and reduces macrophage/neutrophil infiltration, inflammation, oxidative stress and iron overload in IR-stressed livers. WT and Caspase 6.KO mice were used to establish liver IR models. Samples were harvested after 90 min ischemia and 6 h of reperfusion, N = 6/group. A Liver Caspase 6 expression was evaluated by Western blot assay, N = 6/group. B Serum ALT/AST level was detected in ischemia livers; (C) Representative H&E staining and Suzuki’s score in ischemic liver tissue. scale bar: 100 μm, N = 6/group. D Immunofluorescence staining and quantification of CD11b + macrophages in ischemia livers, scale bar: 50 μm, N = 4/group. E IHC staining and quantification of Ly6G + neutrophiles in ischemia livers, scale bar: 50 μm, N = 4/group. F Detection of cytokines IL-1β, TNF-α and CXCL-10 by qRT-PCR in ischemic livers, N = 6/group. Lipid peroxidation by using 4-HNE staining, scale bar: 50 μm, N = 6/group (G) and colorimetric assay, N = 6/group (H); (I) Non-heme iron profiles in liver tissues were measured by colorimetric assay, N = 6/group. All data represent the mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001