Fig. 1

IR induced Caspase 6 expression correlates positively with liver injury in human and mice biopsies. Liver samples were harvested from patients during hepatectomy (after hepatic portal vein occlusion). A H&E staining and Suzuki’s score in High Caspase 6 and Low Caspase 6 groups using the median Caspase 6/β-actin as cutoff value, scale bar: 100 µm, N = 5/group. B Serum ALT/AST was positively correlated with Caspase 6/β-actin by qRT-PCR assay at the first day postoperatively (POD1), N = 15/group. C The level of ALT/AST in High Caspase 6 and Low Caspase 6 groups, N = 15/group. D Serum ALT/AST levels of patients in 7 days after hepatectomy, N = 15/group. E–F IL-1β IHC staining and CD11b IF staining in human liver biopsies, scale bar: 50 µm, N = 5/group. G qRT-PCR analysis of Caspase 6 in livers induced by IR, N = 5/group. H WB analysis of Caspase 6 in mice ischemic livers, N = 4/group. I WB-assisted Caspase 6 expression profile in Kupffer cells and hepatocytes isolated from mice ischemic livers, N = 6/group. All data represent the mean ± SD, *p < 0.05, **p < 0.01