Fig. 2

Function of trophoblast-derived exosomes on HGECs. A Confocal microscopy showed that PKH67-labelled exosomes were internalized by HGECs, Scale: 100 μm. B and C EdU staining was used to examine proliferation after exosomes (100 μg/ml) treatment, Scale: 50 μm. D and F The reaction of cultured HGECs to exosomes (100 μg/mL) was examined in the angiogenesis assay, Scale: 200 μm. E and G Transwell experiments were used to examine HGECs migration after incubation with exosomes (100 μg/mL), Scale: 200 μm. H and I The endothelial monolayer barrier function of cells was tested in the model after treatment with exosomes (100 μg/ml). J Western blot analysis of the expression of VE-cadherin and Occludin in exosomes. K qRT-PCR of the expression of VE-cadherin and Occludin in exosomes. Values are presented as means ± SD, ***P < 0.001, ****P < 0.0001