Fig. 4
From: Reciprocal regulation of TWIST1 and OGT determines the decitabine efficacy in MDS/AML
Inhibition of O-GlcNAcylation on TWIST1 binding to CBLC. A The identified proteins related to ubiquitination analyzed by integrated annotations for Ubiquitin and Ubiquitin-like Conjugation Database (iUUCD, http://iuucd.biocuckoo.org/). B The binding of CBLC and TWIST1 detected by Co-IP. Normal mouse IgG was used as a negative control. C Duo-link proximity ligation assay for interaction between CBLC and TWIST1 in HEK-293 T-WT and HEK-293 T-S31A. Red spot represents for the interaction. Nuclei was stained with DAPI (blue). Cytoskeleton was stained with phalloidine (green). Scale bar = 2 μm. D Western blotting analysis of interaction between CBLC and TWIST1 in KG1a-TWIST1 cells under 10 μg/mL OSMI-1 treatment for 48 h. E Western blotting analysis of interaction between CBLC and TWIST1 in HEK-293 T-WT and HEK-293 T-TWIST1-S31A cells. F Western blotting analysis of interaction between CBLC and TWIST1 in KG1a-TWIST1 under 20 ng/mL DAC treatment for 96 h