Fig. 3
From: Reciprocal regulation of TWIST1 and OGT determines the decitabine efficacy in MDS/AML
Effect of O-GlcNAcylation at Ser 31 on TWIST1 stability. A O-GlcNAclylated GST-TWIST1 in vitro. B O-GlcNAcylation level on WT and site-mutated TWIST1 detected by IP/Western blotting. C TWIST1 level in HEK-293 T cells transfected with WT or mutation TWIST1. D TWIST1 level in HEK293T-WT or HEK-293 T-S31A after 1 μM CHX treatment for indicated times. E Ubiquitination of TWIST1 in HEK293T-WT or HEK-293 T-S31A cells. F TWIST1 level in HEK293T-WT or HEK-293 T-S31A cells after 5 μM MG132 or 0.5 μM Baf-1 treatment for 8 h