Involvement of the tumour necrosis factor receptor system in glioblastoma cell death induced by palbociclib-heptamethine cyanine dye conjugate

Table 5 Trafficking summary data for TNFR1 in response to 1, in the absence or presence of vesicle trafficking inhibitor, BFA, and protein synthesis inhibitor, CHX. E_max expressed as a percentage of vehicle. Parameters are derived from at least three independent patient-derived GBM cases. ns = p > 0.05, p < 0.01, * p < 0.001 relative to 1 with vehicle, One-way ANOVA

1-induced anterograde trafficking of TNFR1 (12 h)
	+ Vehicle	+ BFA	+ CHX
EC ₅₀ (nM) [±SEM]	63.3 [1.5]	481.3 [12.9]	779.8 [293.2]
pEC ₅₀ (log ₁₀ , M) [±SEM]	7.20 [0.01]	6.32 [0.01]***	6.14 [0.17]**
E _max (nM)[±SEM]	4184 [2348]	3835 [2330]^ns	3559 [1975]^ns
1-induced total TNFR1 expression (12 h)
EC ₅₀ (nM) [±SEM]	44421.7 [30.05]	122.9 [23.09]	1154 [15.6]
pEC ₅₀ (log ₁₀ , M) [±SEM]	6.38 [0.03]	6.92 [0.08]*	5.94 [0.01]*
E _max (nM)[±SEM]	6625 [265]	8994 [203]**	5943 [307]**

ISSN: 1478-811X