Fig. 8

Oleic acid is associated with AMPK (Ser496) phosphorylation in platelets. A-B Washed human platelets are pretreated with oleic acid (0 µM, 4 µM, 10 µM), stimulated with collagen, and subsequently lysed with lysis buffer, followed by immunoblotting using phospho-AMPK (Ser496) antibody; N = 5. C Sequence alignment analysis of S496 of AMPKα1 in Homo sapiens, Mus musculus, and Rattus norvegicus D-E Immunofluorescence images of actin in αIIbβ3-CHO cells spreading on immobilized fibrinogen in the absence or presence of oleic acid; N = 5. F αIIbβ3-CHO cells are lysed with lysis buffer, followed by immunoblotting with phospho-AMPK (Ser496). G-H Immunofluorescence staining of actin in αIIbβ3-CHO cells transfected with AMPKα1-S496-flag or AMPKα1-S496A-flag plasmids spreading on immobilized fibrinogen; N = 6. Data are presented as mean ± standard error of mean; one-way analysis of variance; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, NS indicates no statistical significance