Fig. 7
From: The mechanism of oleic acid inhibiting platelet activation stimulated by collagen
Oleic acid inhibits CaMKKβ/AMPK signal transduction. A-B Washed human platelets are pretreated with oleic acid (0 µM, 4 µM, 10 µM), stimulated with collagen, and subsequently lysed with lysis buffer, followed by immunoblotting using phospho-AMPK (Thr172) and ACC (Ser79) antibody; N = 5. C-F Western blotting is performed to assess the phosphorylation levels of CaMKKβ, Cofilin (Ser3), and VASP(Thr278), N = 5. Data are presented as mean ± standard error of mean; one-way analysis of variance; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, NS indicates no statistical significance