Fig. 5

Effect of oleic acid in vivo. A-B Carotid artery occlusion time is measured after ferric chloride-induced injury using a flow probe. The occlusion time of the oleic acid (5 mg/kg) group is compared with that of the vehicle group. C Representative histological images of thrombi stained with hematoxylin and eosin are shown. D-F (D) Venous thrombus formation in mice treated with vehicle or oleic acid (5 mg/kg) (vehicle, 25 out of 31; oleic acid, 21 out of 30); Chi-square test; Furthermore, E length and F weight of thrombi are compared between the control and experimental groups. G The tail bleeding assay is performed in mice treated with oleic acid (5 mg/kg) or vehicle. The tail bleeding time is determined as the time taken for the initial cessation of bleeding; N = 8. H Mice are injected with oleic acid (5 mg/kg) or the solvent through the tail vein. Thirty minutes later, the whole blood is collected from the orbit, and platelet counts of mice are measured using a blood cell counter (vehicle, N = 7; oleic acid, N = 5). I-K Mice are injected with oleic acid (5 mg/kg) or the solvent through the tail vein. The effect of oleic acid on plasma coagulation in mice is detected using activated partial thromboplastin time, prothrombin time, and fibrinogen (vehicle, N = 6; oleic acid N = 7). Data are presented as mean ± standard error of mean; unpaired t-test; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, NS indicates no statistical significance