Fig. 4

Oleic acid inhibits intracellular Ca²⁺ concentrations in platelets. A-C Collagen (6 µg/mL) induced Ca²⁺ mobilization, as determined by the Fluo-3-AM fluorescent signal, which is monitored over time using flow cytometry in platelets pretreated with oleic acid or vehicle. A Summary data of the fluorescent signal are shown; N = 5. B Total Ca²⁺ influx (area under the curve) and (C) maximum Ca²⁺ signal (peak height) quantified using the kinetics curve; N = 5. Data are presented as mean ± standard error of mean; one-way analysis of variance; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, NS indicates no statistical significance