Fig. 3

Oleic acid inhibits integrin αIIbβ3 outside-in signaling. A-B Washed human platelets exposed to immobilized fibrinogen for 60 min are incubated with various concentrations of oleic acid or vehicle. A Representative images and (B) summary data of the mean platelet surface area are shown; N = 5. C-D Western blotting is performed to assess the phosphorylation levels of integrin 𝛽3 (Tyr773); N = 5. E-F Washed human platelets are incubated with oleic acid or vehicle, and the clot retraction assay is performed; N = 5. G-H Washed human platelets are labelled with Cell Trace Calcein Green and then incubated with vehicle or oleic acid for 5 min. Representative images of surface coverage are shown under arterial shear rates(2000/s). Scale bar = 50 μm; N = 8. Data are presented as mean ± standard error of mean; one-way analysis of variance; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, NS indicates no statistical significance