Fig. 2

Oleic acid inhibits platelet granule secretion and the αIIbβ3 signaling pathway. A Washed human platelets are stimulated with collagen in the presence of various concentrations of oleic acid or vehicle at 37 °C and then labelled with fluorescein isothiocyanate-conjugated P-selectin. P-selectin exposure is determined using flow cytometry; N = 5. B Washed human platelets are stimulated with collagen in the presence of various concentrations of oleic acid or vehicle at 37 °C and then labelled with fluorescein isothiocyanate-conjugated PAC-1 antibodies. PAC-1 binding is determined using flow cytometry; N = 5. C-D Washed human platelets are pretreated with various concentrations of oleic acid or vehicle at 37 °C and then stimulated with collagen. The concentration of ATP is assessed using Chrono-lume; N = 5. E Cytotoxicity of oleic acid, as measured using the lactate dehydrogenase assay, is determined in washed platelets treated with oleic acid or vehicle; Max group is the addition of lysate; N = 6. Data are presented as mean ± standard error of mean; one-way analysis of variance; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, NS indicates no statistical significance